The general hybridoma antibody cloning protocol and antibody sequencing protocol seem very straightforward: RNA extraction, reverse transcription to cDNA, nested PCR amplifications, cloning of PCR products, sequencing, and analysis of sequences. However, it has taken scientists a long time to optimize a variety of parameters in the protocols, especially V-gene specific primers and antibody sequence […]
Sympros organizes Antibody Production and Applications Resources related resource and information for Antibody Production and Applications Resources freelancers, studios, teams, and companies (collectively referred to as "professionals") and clients.
Sympros cooperates with a large number of domestic and international Antibody Production and Applications Resources professionals to deliver the most cost-effective Antibody Production and Applications Resources services to individuals and organizations in different stages of development, especially small and medium-sized businesses.
Antibody Production and Applications Resources professionals post and display their most comprehensive archive of information (business cards + resumes + services + public information) in the Antibody Production and Applications Resources Professional subcategory. Based on such information, clients and colleagues can know and contact Antibody Production and Applications Resources professionals. If there is any dispute between client and professional in private contact or any resulted loss, Sympros takes no responsibility.
Professionals can not only view Antibody Production and Applications Resources projects under the bidding project category of Antibody Production and Applications Resources service, but also contact Sympros for information about hundreds of Antibody Production and Applications Resources projects from US and international clients, which are not displayed in public. Some projects are posted for bidding, and some are allocated directly to the cost-effective professionals that we know well. Project co-operation is the best way to get to know professionals well, and the best way for professionals to know us better.
Similar to process of hybridoma or clonal B cell antibody cloning and sequencing, the work flow of single B cell antibody cloning and sequencing is: separation of peripheral blood mononuclear cells (PBMC) from blood, staining of PBMC with the B cell selective marker (CD19 antibody), sorting of stained B cells by flow cytometry, total RNA […]
Two mainstream strategies have been used to sequence antibody repertoires. The classical strategy is to design primers to cover as many functional V-genes as possible and create a diverse antibody libraries. The modern strategy is to use high-throughput next generation sequencing (NGS) to “deeply” sequencing of binding populations. The abundance of antibody mRNA can also […]
The key for successful scFv phage library construction is efficient two-step PCR amplification of heavy chain (VH) and ligh chain (VL) variable regions. scFv phage library construction and scFv phage library screening protocols from public literatures: – Efficient amplification of light and heavy chain variable regions and construction of a non-immune phage scFv library. […]